官方微信|手机版

产品展厅

产品求购企业资讯会展

发布询价单

化工仪器网>产品展厅>试剂标物>行业专用试剂>生化与分子生物学用试剂> 人瘦素(LEP)ELISA Kit

分享
举报 评价

人瘦素(LEP)ELISA Kit

具体成交价以合同协议为准

联系我们时请说明是化工仪器网上看到的信息,谢谢!


上海希美化学有限公司有限公司是一家专业代理销售生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器的高科技企业。产品涉及分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域,同时我们提供专业的生物技术服务。
     
公司一方面注意跟踪世界范围分子生物学、细胞生物学等生物学科的发展前沿,努力将欧美专业生产厂家的具有*水平的产品*给各类研究人员;另一方面也非常重视自主产品研究和开发,推出了一系列具有竞争力的产品和服务。公司的经营原则是质量*,诚信*,品牌*,以质量是企业的生命,诚信是经营的资本为警醒 ,严把所售出商品的质量关。公司客户遍布大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位。
     
公司在重视产品质量的同时,也建立了一套集技术支持、物流、售后服务等多个部门的*的服务体系,努力把我们方便、快捷、周到的服务提供给每一个客户。主要代理产品:试剂盒:ELISA试剂盒、免疫组化试剂盒、分子生物学试剂盒、细胞凋亡试剂盒。

ELISA KIT :CUSABIO品牌涵盖37个种属多达五千多种的Elisa试剂盒;拥有广泛的细胞因子、生长因子、趋化因子、激素、酶、病毒抗原和其他许多重组蛋白的蛋白表达系统。

化学试剂:*生化试剂、进口分装生化试剂、国产生化试剂。

抗体:进口原装抗体、进口分装抗体、单克隆抗体、多克隆抗体、一抗、二抗等等 进口代理品牌:
Sigma Peprotech Prospec eBioscience 、伯乐、 Invitrogen MBI Hyclone CaymanCUSABIO Wako SantaCruzepitomicsGibcoR&DLincoabgentMilliporeInnova BiosciencesCstAbcam

ELISA KIT,酶免试剂盒,酶联免疫试剂盒,重组蛋白,抗体

产品名称: 人瘦素(LEP)ELISA Kit
 
 英文名称: Human Leptin,LEP ELISA kit
 
 货号: CSB-E04649h
 
 规格: 96T
 
 种属: Human
 
 待测物名称: leptin
 
 缩写: LEP
 
 样本类型: serum, plasma, cell culture supernates, tissue homogenates
 
 检测范围: 6.25 pg/ml-400 pg/ml
 
 灵敏度: 1.56 pg/ml
 
 反应时间: 1-5h
 
 所需样本体积: 50-100ul
 
 检测波长: 450 nm
 
 用途: For research use only. Not for diagnostic use.
 
 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEP bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性: This assay has high sensitivity and excellent specificity for detection of Human LEP. No significant cross-reactivity or interference between Human LEP and analogues was observed. 
 精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the LEP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
相关产品:
 
产品名称 英文名称 货号 规格
 猴子瘦素(LEP)ELISA Kit  Monkey Leptin,LEP ELISA Kit  CSB-E14936Mk  96T 
        
 鱼(鲑鱼)瘦素(LEP) ELISA kit  Fish (Salmon) leptin (LEP) ELISA kit  CSB-EL012870FI  96T 
        
 猪瘦素(LEP)ELISA Kit  Pig Leptin,LEP ELISA Kit  CSB-E06815p  96T 
        
 小鼠瘦素(LEP)ELISA Kit  Mouse Leptin,LEP ELISA kit  CSB-E04650m  96T 
        
 牛瘦素(LEP)ELISA Kit  Bovine Leptin,LEP ELISA Kit  CSB-E06771b  96T 
        
 大鼠瘦素(LEP)ELISA kit  Rat Leptin,LEP ELISA kit  CSB-E07433r  96T 
        
 仓鼠瘦素(LEP) ELISA kit  Hamster leptin (LEP) ELISA kit  CSB-EL012870HA  96T 
        
 小尾寒羊瘦素(LEP)ELISA kit  Small Tailed Han Sheep Leptin,LEP ELISA kit  CSB-E12839Sh  96T 
        
 Sheep Leptin(LEP) ELISA kit  Sheep Leptin(LEP) ELISA kit  CSB-EL012870SH  96T 
        
 兔子瘦素(LEP)ELISA Kit  rabbit Leptin,LEP ELISA Kit  CSB-E06971Rb  96T 
        
 Horse Leptin(LEP) ELISA kit  Horse Leptin(LEP) ELISA kit  CSB-EL012870HO  96T 
        
 山羊瘦素(LEP)ELISA kit  Goat Leptin,LEP ELISA kit  CSB-E12834G  96T 
        
 Dog Leptin(LEP) ELISA kit  Dog Leptin(LEP) ELISA kit  CSB-EL012870DO  96T 
        
 鸡瘦素(LEP)ELISA kit  Chicken Leptin,LEP ELISA kit  CSB-E14082C  96T 
        
 Cat Leptin(LEP) ELISA kit  Cat Leptin(LEP) ELISA kit  CSB-EL012870CA  96T 
        
抗体 
产品名称 货号 规格
 Rabbit anti-human Leptin,LEP polyclonal Antibody  CSB-PA012870GA01HU  150ul 
      
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Meleagris gallopavo Leptin(LEP)  CSB-EP012870MQV  1mg  E.coli 
        
 Recombinant Meleagris gallopavo Leptin(LEP)  CSB-YP012870MQV  1mg  Yeast 
        
 Recombinant Pan troglodytes Leptin(LEP)  CSB-EP012870EQV  1mg  E.coli 
        
 Recombinant Pan troglodytes Leptin(LEP)  CSB-YP012870EQV  1mg  Yeast 
        
 Recombinant Dog Leptin(LEP)  CSB-EP012870DO  1mg  E.coli 
        
 Recombinant Dog Leptin(LEP)  CSB-YP012870DO  1mg  Yeast 
        
 Recombinant Chicken Leptin(LEP)  CSB-EP012870CH  1mg  E.coli 
        
 Recombinant Chicken Leptin(LEP)  CSB-YP012870CH  1mg  Yeast 
        
 Recombinant Rat Leptin(Lep)  CSB-EP012870RA  1mg  E.coli 
        
 Recombinant Rat Leptin(Lep)  CSB-YP012870RA  1mg  Yeast 
        
 Recombinant Bovine Leptin(LEP)  CSB-EP012870BO  1mg  E.coli 
        
 Recombinant Bovine Leptin(LEP)  CSB-YP012870BO  1mg  Yeast 
        
 Recombinant Human Leptin(LEP)  CSB-EP012870HU  1mg  E.coli 
        
 Recombinant Human Leptin(LEP)  CSB-YP012870HU  1mg  Yeast 
        
 Recombinant Mouse Leptin(Lep)  CSB-EP012870MO  1mg  E.coli 
        
 Recombinant Mouse Leptin(Lep)  CSB-YP012870MO  1mg  Yeast



化工仪器网

采购商登录
记住账号    找回密码
没有账号?免费注册

提示

×

*您想获取产品的资料:

以上可多选,勾选其他,可自行输入要求

个人信息: