15032350 illumina NXTR XT样本制备试剂盒 *
- 公司名称 上海易汇生物科技有限公司
- 品牌 illumina/美国因美纳
- 型号 15032350
- 产地 美国进口
- 厂商性质 生产厂家
- 更新时间 2021/11/16 14:38:15
- 访问次数 1138
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供货周期 | 现货 | 规格 | 盒 |
---|---|---|---|
货号 | 15032350 | 应用领域 | 医疗卫生,化工,生物产业,制药,综合 |
主要用途 | 基因测序 |
illumina NXTR XT样本制备试剂盒 *
illumina NXTR XT样本制备试剂盒 *
illumina 15032350
illumina 15032350 NXTR XT样本制备试剂盒
NXTR XT样本制备试剂盒(24样本 第一盒)
Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354
NXTR XT标签试剂盒V2(套件A 96标签 192样本)15052163
Next-Generation sequencing
Total RNA was extracted from each patient’s plasma sample, and cDNA synthesis followed. The QIAamp Viral RNA Mini Kit (Cat. #52904, Qiagen, Gaithersburg, MD) and the SuperScript�6�4 IV First-Strand Synthesis System (Cat. # 18091050, Invitrogen, Carlsbad, CA) were used respectively and according to manufacturers’ instructions. Multiple sets of HIV-1 specific primer pairs were used to target and amplify using polymerase-chain-reaction (PCR) the protease (PR), reverse transcriptase (RT), integrase (int), and envelope (env) HIV-1 genes (~43% of genome)36. Library preparation was completed with Nextera XT Library Prep (Cat. # 15032350, Illumina, Dan Diego, CA). Samples were then sequenced on eight runs on an Illumina MiSeq platform using the MiSeq v2 (300 cycles) chemistry (Cat. # MS-102–2002, Illumina). Both library prep and sequencing were completed according to the manufacturer’s instructions. All DNA sequence files are available from the GenBank database under SRA accession: PRJNA517147.
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