RealTime ready Cell Lysis 罗氏细胞裂解试剂盒

RealTime ready Cell Lysis Kit   实时准备细胞 裂解试剂盒

Reagent for lysing cells prior to two-step real-time RT-PCR applications.

用于两步实时RT-PCR应用前裂解细胞的试剂。

For life science research only. Not for use in diagnostic procedures.

仅供生命科学研究使用。不用于诊断程序。

Overview概述：

Increase convenience:

Replace cumbersome multi-step RNA purification protocols with a simple one-step cell lysis procedure.

增加便利性：

用简单的一步细胞裂解程序取代繁琐的多步 RNA 纯化程序。

Improve time to result:

Lyse samples in just 5 minutes at room temperature without further steps.

缩短获得结果的时间：

在室温下仅需 5 分钟即可裂解样品，无需进一步操作。

Reduce intermediate steps:

Obtain faster, high-quality results by performing DNase treatment during cDNA synthesis.

减少中间步骤：

通过在 cDNA 合成过程中进行 DNase 处理，获得更快、高质量的结果。

Benefit from broad applicability:

Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats with or without automation.

受益于广泛的适用性：

在 96 至 1536 孔格式的实时 PCR 应用中，无论是否采用自动化，都可以将 3 到 30,000 个培养细胞放大。

Contents 内容

1. Ready-to-use Lysis Buffer (20 mL)

   即用型裂解缓冲液 (20 mL)

• Protector RNase Inhibitor (250 µL - 40 U/µL)

   保护器核糖核酸酶抑制剂 (250 µL - 40 U/µL) 

• Thermolabile Nuclease (250 µL)

   耐热核酸酶 (250 µL)

Application应用：

The RealTime ready Cell Lysis Kit is designed for rapid, high-performance gene expression studies using cultured cells in two-step, real-time RT-PCR applications. The kit uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly in cDNA synthesis, eliminating the time-consuming RNA purification step. Finally, the cDNA can be analyzed in real-time PCR experiments at any throughput (e.g., LightCycler® 480 Instrument, 96- and 384-well; LightCycler® 1536 Instrument, 1536-well). Due to the user-friendly packaging and easy-to-use protocol, the RealTime ready Cell Lysis Kit can be easily adapted to all experimental and throughput requirements. In addition, the kit easily integrates into all manual and automated cell-based gene expression workflows.

实时准备细胞裂解试剂盒设计用于在两步实时 RT-PCR 应用中使用培养细胞进行快速、高性能的基因表达研究。该试剂盒采用*一步式方案，可在室温下 5 分钟内裂解 3 至 30,000 个细胞。所得裂解物可直接用于 cDNA 合成，省去了耗时的 RNA 纯化步骤。最后，可以在任何通量的实时 PCR 实验中分析 cDNA（例如，  LightCycler ®  480 仪器，96 孔和 384 孔；LightCycler ® 1536 仪器，1536 孔）。由于用户友好的包装和易于使用的协议，RealTime ready Cell Lysis Kit 可以轻松适应所有实验和通量要求。此外，该试剂盒可轻松集成到所有手动和自动基于细胞的基因表达工作流程中。