手持式叶绿素荧光仪---FluorPen FP110

       手持式叶绿素荧光仪---FluorPen FP110用于实验室、温室和野外快速测量植物叶绿素荧光参数，具有便携性强、精度高、性价比高等特点；双键操作，具图形显示屏，内置锂电和数据存储，广泛应用于研究植物的光合作用、胁迫监测、除草剂检测或突变体筛选，还可用于生物毒理的生物检测，如通过不同植物对土壤或水质污染的叶绿素荧光响应，找出敏感植物作为生物传感器用于生物检测。FP110配备多种叶夹型号，用于不同的样品与研究。

应用领域

       手持式叶绿素荧光仪---FluorPen FP110适用于光合作用研究和教学，植物及分子生物学研究，农业、林业，生物技术领域等。研究内容涉及光合活性、胁迫响应、农药药效测试、突变筛选等。

• 植物光合特性研究
• 光合突变体筛选与表型研究
• 生物和非生物胁迫的检测
• 植物抗胁迫能力或者易感性研究
• 农业和林业育种、病害检测、长势与产量评估
• 除草剂检测
• 教学

功能特点

• 结构紧凑、便携性强，LED光源、检测器、控制单元集成于仅手机大小的仪器内，重量仅188g
• 功能强大，是叶绿素荧光技术的高新结晶产品，具备了大型荧光仪的所有功能，可以测量所有叶绿素荧光参数
• 内置了所有通用叶绿素荧光分析实验程序，包括3套荧光淬灭分析程序、3套光响应曲线程序、OJIP快速荧光动力学曲线等
• 高时间分辨率，可达10万次每秒，自动绘出OJIP曲线并给出26个OJIP–test参数
• FluorPen专业软件功能强大，可下载、展示叶绿素荧光参数图表，也可以通过软件直接控制仪器进行测量
• 具备无人值守自动监测功能
• 内置蓝牙与USB双通讯模块，GPS模块，输出带时间戳和地理位置的叶绿素荧光参数图表
• 配备多种叶夹型号：固定叶夹式（适于实验室内暗适应或夜间快速测量）、分离叶夹式（适用于野外暗适应测量）、探头式（透明光纤探头，具备叶片固定装置，用于非接触性测量监测或光适应条件下的叶绿素荧光监测）、用户定制式等
• 可选配野外自动监测式荧光仪，防水防尘设计

测量程序与功能

• Ft：瞬时叶绿素荧光,暗适应完成后Ft＝F₀
• QY：量子产额，表示光系统II 的效率，等于Fv/Fm(暗适应状态)或ΦPSII (光适应状态)。
• OJIP：快速荧光动力学曲线，用于研究植物暗适应后的快速荧光动态变化
• NPQ：荧光淬灭动力学曲线，用于研究植物从暗适应到光适应状态的荧光淬灭变化过程。
• LC：光响应曲线，用于研究植物对不同光强的荧光淬灭反应。
• PAR：光合有效辐射，测量环境中植物生长可以利用的400-700nm实际光强（限PAR型号）。

应用案例

2017年4月，美国国家航空*（NASA）新一代*植物培养器（Advanced Plant Habitat，APH）搭载联盟号MS-04货运飞船抵达空间站。宇航员使用FluorPen手持仪叶绿素荧光仪在其中开展植物生理学及太空食物种植（growth of fresh food in space）的研究。

参考文献

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附：OJIP参数及计算公式

Bckg = background

Fo: = F50µs; fluorescence intensity at 50 µs

Fj: = fluorescence intensity at j-step (at 2 ms)

Fi: = fluorescence intensity at i-step (at 60 ms)

Fm: = maximal fluorescence intensity

Fv: = Fm - Fo (maximal variable fluorescence)

Vj = (Fj - Fo) / (Fm - Fo)

Fm / Fo = Fm / Fo

Fv / Fo = Fv / Fo

Fv / Fm = Fv / Fm

Mo = TRo / RC - ETo / RC

Area = area between fluorescence curve and Fm

Sm = area / Fm - Fo (multiple turn-over)

Ss = the smallest Sm turn-over (single turn-over)

N = Sm . Mo . (I / Vj) turn-over number QA

Phi_Po = (I - Fo) / Fm (or Fv / Fm)

Phi_o = I - Vj

Phi_Eo = (I - Fo / Fm) . Phi_o

Phi_Do = 1 - Phi_Po - (Fo / Fm)

Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms)

ABS / RC = Mo . (I / Vj) . (I / Phi_Po)

TRo / RC = Mo . (I / Vj)

ETo / RC = Mo . (I / Vj) . Phi_o)

DIo / RC = (ABS / RC) - (TRo / RC)