8046 MicroVue 25-OH Vitamin D EIA

产品描述

The MicroVue 25-OH Vitamin D EIA is an immunocapture assay for the determination of total 25-OH Vitamin D in human serum.

Vitamin D is a group of fat-soluble secosteroids which are produced in the skin of vertebrates after exposure to ultraviolet light from the sun, available from artificial sources, or occur naturally in a small range of foods. Total 25-OH Vitamin D is a combined measurement of two major forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol); known collectively as calciferol. When synthesized in the kidneys, calciferol circulates as a hormone; regulating, among other things, the concentration of calcium and phosphate in the bloodstream, promoting the healthy mineralization, growth and remodeling of bone.

性能特点

方法：免疫捕获

分析物：25-OH 维生素 D2 和 D3

样本：血清

样本体积：50 µL

检测限：1.5 ng/mL

动态范围：0-133 ng/mL

运行内精密 CV：2.7%-5.7%

两次运行之间的精确 CV：4.3%-4.7%

孵育时间：3 小时

参考文献

1. Zerwekh, J.E. Blood biomarkers of Vitamin D status. Am. J. Clin. Nutr. 2008; 87(suppl):1087S-1091S.

2. Holick, M.F. Resurrection of Vitamin D deficiency and rickets. J. Clin. Invest. 2006; 116:2062-2072.

3. Heaney, R.P. VitaminD: how much do we need and how much is too much. Osteoporos. Int. 2000; 11(7)

553-555