ICP9947 Ochratoxin A Antibody, HRP

Product Description

This antibody was developed using ochratoxin A (OA)-KLH conjugates, linked via its free carboxyl group. The antibody was affinity purified with an OA-agarose affinity column. The purified antibody was chemically conjugated to HRP. It could be utilized for direct competitive detection and quantization of the food-borne mycotoxin, ochratoxin A.

Direct competitive inhibition ELISA using immobilized anti-OA (1.5 µg/mL), and OA-labelled HRP (50 ng/mL). 50% inhibition occurred at 25 ng/mL of free ochratoxin A in non-optimized conditions.

Species

Rabbit

Formulation

0.25 mg/mL in stabilizing buffer

Immunogen

Pure ochratoxin A linked to KLH, via the free carboxyl group of OA using the NHS-active ester method.

Purification

Antigen-specific affinity chromatography

Specificity

The antibody recognizes ochratoxin A (*), C (400%), B (5%), and <1% of ochratoxin alpha.

Applications

ELISA,  affinity column enrichment

Note: For better ELISA results, use a different method than the NHS-active ester method to prepare the paired antigen, such as the OA-OV with EDC method.

Scientific Description

Ochratoxin A is a food borne mycotoxin which is carcinogenic and nephropathic to both humans and animals. Contamination with OA in food may be harmful to humans and animals.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is tweleve months from date of shipping if stored properly.

Product Specific References

1. 1. Sens. Actuator B-Chem. 2015. 221: 777-783. doi10.1016/j.snb.2015.07.007
2. 2. Sens. Actuator B-Chem. 2016. 235: 554-562. doi:10.1016/j.snb.2016.05.124
3. 3. Biosensors and Bioelectronics. 2016. 87. 823-831. doi:http://dx.doi.org/10.1016/j.bios.2016.09.041
4. 4. Sensors and Actuators B: Chemical. 2016. 235. 554-562 doi:http://dx.doi.org/10.1016/j.snb.2016.05.124