ICP9705 羊抗鼠IgG免疫亲和填料Goat Anti-mouse IgG,Agarose

Product Description

Monoclonal antibody production using cell culture systems somtimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from both mouse serum, ascites and the supernatant, of the cell culture media, without pulling-down boving IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product as been successfully used to replace protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity > 10 mg.mL.

Formulation

1 mL beads in 2 mL glycerol

Ligand Immobilized

40 mg specific affinity-purified anti-mouse IgG/mL

Binding Capacity

>10 mg/mL of mouse IgG

Applications

Affinity chromatography ; Immunoprecipitation

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if stored properly.

Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.

Fraction 1: 0.8 mg/mL

Fraction 2: 2.5 mg/mL

IgG Std: 0.31 - 5 mg/mL