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化工仪器网>产品展厅>试剂标物>行业专用试剂>生化与分子生物学用试剂>ICP9903/9820/9902 磷酸化丝氨酸抗体生物素标记/苏氨酸抗体生物素标记/酪氨酸抗体生物素标记

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ICP9903/9820/9902 磷酸化丝氨酸抗体生物素标记/苏氨酸抗体生物素标记/酪氨酸抗体生物素标记

参考价 ¥ 3880
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南宁市蓝光生物技术有限公司成立于2008,坐落在广西南宁市*开发区。是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有*科学家和设备的实验室提供。

蓝光生物的产品和服务包括多肽合成、抗体制备、基因克隆和蛋白表达、免疫学和细胞生物学相关试剂以及技术支持等。目前,公司已开发出近几百种抗体产品,包括:用于蛋白组学和药物筛选的甲基化、乙酰化、磷酸化等翻译后修饰蛋白质组学的抗体和填料;固相非放射性同位素蛋白激酶活性测试盒(PKC,PKA,AKT,SGKS6K蛋白激酶测试盒)以及用于基础生物医学研究的各种抗体、各种广泛应用于科研的标记第二抗体。这些产品可应用于染色体修饰、细胞周期、细胞周期检验点、细胞凋亡、核因子、神经因子、细胞因子、酪氨酸激酶、翻译调控因子、淋巴细胞信号传导因子、糖代谢等细胞信号传导通路的科学研究。其中,用于药物筛选和基础科研的非放射性同位素蛋白激酶活性测试盒属世界*;自主研发的甲基化、乙酰化、磷酸化特异性抗体及亲和色谱填料等翻译后修饰蛋白质组学抗体和填料等系列产品国内外。客户用我们的产品进行科学研究,论文发表在SCIENCENATURECELL等高质量的学术杂志上。

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Phospho-Serine Antibody, Biotin

Catalog #:

ICP9903

 

 

 

 

 

 

 

 

 

Product

Description

The affinity purified rabbit anti-phospho-serine antibodies are conjugated to d-biotin. The conjugates are useful for detection, identification, and quantization of serine-phosphorylated proteins and peptides.

Species

Rabbit

Formulation

250 µg/mL in PBS, 40% glycerol

Immunogen

Phospho-serine-KLH conjugates

 

Purification

The antibody was purified with phospho-serine on agarose

 

Conjugation

Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin)

 

Specificity

The antibody is pan-specific against phospho-serine. It does not cross react with phospho-tyrosine but does react slightly with phospho-threonine.

 

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)

 

Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to form phospho-serine, which is responsible for various signalling processes in eukaryotes. [1]

 

Storage & Stability

 

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC.  Expiration date is one year from date of shipping if properly stored.

 

Product Specific References

1. Curr. Opin. Cell. Bio. 2001. 13(2): 131-138. doi:10.1016/S0955-0674(00)00189-7

 

 

Phospho-Threonine Antibody, Biotin

Catalog #:

ICP9820

Product Description

Affinity purified rabbit anti-phospho-threonine antibodies are conjugated to d-biotin via N-acetylation. The conjugates are useful for detection, identification and quantization of threonine-phosphorylated proteins and peptides.

 

 

 

 

Species

Rabbit

 

 

Formulation

250 µg/mL in PBS, 50% glycerol

 

 

Immunogen

Phospho-threonine-KLH conjugates and phosvitin

 

 

Purification

The antibody was purified on phospho-threonine agarose.

 

 

Conjugation

Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin)

 

 

Specificity

The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.

 

 

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)

 

 

Scientific Description

Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate threonine to form phospho-threonine which is considered a key step in the regulation of enzymatic activities such as signal transduction.

 

 

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

 

 

Product Specific References

  • 1. Betts, M. J. and Russell, R. B. (2003) Amino Acid Properties and Consequences of Substitutions, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867302.ch14

 

 

Phospho-Tyrosine Antibody, HRP

Catalog #:

ICP9902

Product Description

The affinity purified rabbit polyclonal anti-phospho-tyrosine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides.


Immunoprecipitated samples with

 anti-phospho-tyrosine agarose (ICP9804)

Left: Mouse spleen lysate treated with

 complete adjuvant   Right: Mouse spleen

lysate without any treatment   Detection:

Phospho-tyrosine antibody, HRP (ICP9902)

 

 

Species

Rabbit

Formulation

250 µg/mL in HRP stabilizing buffer with 50% glycerol

Immunogen

Iodoacetylphosphotyramine-KLH conjugates

Purification

The antibody was affinity purified with phospho-tyrosine immobilized on agarose, followed by immune affinity absorption with tyrosine on agarose.

Conjugation

Reductive amination; HRP/Ab ratio - 10:1

Specificity

The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.

Applications

ELISA (1:1000); WB (1:1000)

Scientific Description

Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.

 

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

  • 1. J. Bio. Chem. 2006. 281: 37877-37887.doi:10.1074/jbc.M609398200

 



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