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产品概述

Specificity ：THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.

Host Species： Mouse

Immunogen： A synthetic peptide (DYKDDDDK) coupled to KLH

Conjugate ：Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application：Recommended Usage

ELISA ：0.05-0.2 µg/ml

Western Blot： 0.1-1.0 µg/ml

Immunoprecipitation (IP) ：1 µg/ml

Immunocytochemistry/Immunofluorescence (ICC/IF) ：1 µg/ml

Flow Cytometry： 1 µg/ml

产品属性

Form： Lyophilized

Storage Buffer： Lyophilized with PB, pH 6.5, containing 0.02% sodium azide

Reconstitution ：Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Storage Instructions ：The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Purification ：Protein A affinity column

Isotype： Mouse IgG2b,κ

Clonality： Monoclonal

Clone ID ：5A8E5

Note ：GenScript can offer this product per customer's request including product size, buffer components, etc.

技术背景

Target Background： The DYKDDDDK peptide is a small epitope tag which is widely used in expression vectors. The tag does not appear to interfere with the bioactivity or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins

Synonyms： Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.