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ALK/p80间变性淋巴瘤激酶（鼠单克隆抗体）

5A4

广州健仑生物科技有限公司

间变性大细胞淋巴瘤，即是非霍奇金淋巴瘤的一种独立类型，由德国病理学家Stein等于1985年应用Ki-1（CD30）抗体识别，常呈间变性特征，被命名为间变性大细胞淋巴瘤。REAL分类将B细胞表型者归为弥漫性大B细胞性淋巴瘤。目前，ALCL只包括T表型和Null（非T非B）表型。约60%-85%左右ALCL病例表达间变性淋巴瘤激酶（anaplasticlymphomakinase，ALK）融合蛋白，这是由于2号染色体上的ALK基因位点的畸变所致。zui常见的是t（2；5）（p23；q35）而形成融合基因NPM-ALK，它是由位于5号染色体上的核仁磷酸蛋白B23（NPM）基因与位于2号染色体的ALK基因相融合形成，表达融合蛋白为NPM-ALK蛋白；zui近尚有更多的ALK基因与其他基因通过染色体转位或者是染色体的倒转而形成的融合基因被发现，如t（1；2）（q25；p23）所形成的TPM3-ALK基因，t（2；3）（p23；q21）产生的TFG-ALKs基因，TFG-ALKL基因和TFG-ALKxL基因，inv（2）（p23；q35）所形成的ATIC-ALK基因，t（2；17）（p23；q23）形成的CLTCL-ALK基因及t（X；2）（q11；p23）形成的-ALK基因。

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【产品介绍】

细胞定位：细胞浆/细胞核

克隆号：5A4

同型：IgG1

适用组织：石蜡/冰冻

阳性对照：间变性大细胞淋巴瘤/肺腺瘤

抗原修复：热修复（EDTA）

抗体孵育时间：60min

ALK/p80间变性淋巴瘤激酶（鼠单克隆抗体）5A4

检查
1.病原学检查
（1）粪便检查 ①活滋养体检查法 常用生理盐水直接涂片法检查活动的滋养体。急性痢疾患者的脓血便或阿米巴炎病人的稀便，要求容器干净，粪样新鲜、送检越快、越好，寒冷季节还要注意运送和检查时的保温。典型的阿米巴痢疾粪便为酱红色黏液样，有特殊的腥臭味。镜检可见黏液中含较多粘集成团的红细胞和较少的白细胞，有时可见夏科-雷登氏结晶和活动的滋养体。这些特点可与细菌性痢疾的粪便相区别。②包囊检查法 以竹签沾取少量粪样，在碘液中涂成薄片加盖玻片，然后置于显微镜下检查，鉴别细胞核的特征和数目。
（2）阿米巴培养 由于技术操作复杂，需一定设备，且阿米巴人工培养在多数亚急性或慢性病例阳性率不高，似不宜作阿米巴诊断的常规检查。
（3）组织检查 通过乙状结肠镜或纤维结肠镜直接观察黏膜溃疡，并作组织活检或刮拭物涂片，检出率zui高。滋养体的取材必须在溃疡的边缘，钳取后以局部稍见出血为宜。脓腔穿刺液检查除注意性特征外，应取材于脓腔壁部，较易发现滋养体。
2.免疫检查
近年来国内外陆续报告了多种血清学诊断方法，其中以间接血凝（IHA）、间接荧光抗体（IFAT）和酶联免疫吸附试验（ELISA）研究较多，但敏感性对各型病例不同。IHA的敏感较高，对肠阿米巴病的阳性率达98%，肠外阿米巴病的阳性率达95%，而无症状的带虫者仅10%～40%，IFA敏感度稍逊于IHA。EALSA敏感性强，特异性高，有发展前途。近年来，已有报道应用敏感的免疫学技术在粪便及脓液中检测阿米巴特异性抗原获得成功。特别是抗阿米巴杂音瘤单克隆抗体的应用为免疫学技术探测宿主排泄物中病原物质了可靠、灵敏和抗干扰的示踪式具。
诊断
对阿米巴病的诊断，除根据患者的主诉、病史和临床表现作为诊断依据外，重要的是病原学诊断，粪便中检查到阿米巴病原体为惟一可靠的诊断依据。通常以查到大滋养体者作为现症患者，而查到小滋养体或包囊者只作为感染者。
鉴别诊断
阿米巴肠病需和细菌性痢疾、血吸虫病、肠结核、结肠癌、慢性非特异性溃疡性结肠炎等鉴别。
1.细胞性痢疾
起病急，全身中毒症状严重，抗生素治疗有效，粪便镜检和细菌培养有助于于诊断。
2.血吸虫病
起病较缓，病程长，有疫水接触史，肝脾肿大，血中嗜酸粒细胞增多，粪便中可发现血吸虫卵或孵化出毛蚴，肠黏膜活组织中可查到虫卵。
3.肠结核
大多有原发结核病灶存在，患者有消耗性热、盗汗、营养障碍等；粪便多呈黄色稀粥状，带黏液而少脓血，腹泻与便秘交替出现。胃肠道X线检查有助于诊断。
4.结肠癌
患者年龄较大，多有排便习惯的改变，大便变细，有进行性贫血，消瘦。晚期大多可扪及腹块，X线钡剂灌肠检查和纤维结肠镜检查有助于诊断。
5.慢性非特异性溃疡性结肠炎
临床症状与慢性阿米巴病不易区别，但大便检查不能发现阿米巴，且经抗阿米巴治疗仍不见效时可考虑本病。

ALK/p80间变性淋巴瘤激酶（鼠单克隆抗体）5A4

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

【】 
【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

an examination
1. Etiological examination
(1) stool examination ① live trophozoite test commonly used saline direct smear activity of trophozoites. Patients with acute dysentery or pus and blood in patients with amebiasis loose stools, requiring containers clean, fecal samples, inspection faster, the better, the cold season but also pay attention to transport and inspection of the insulation. Typical amebic dysentery faeces are reddish mucus-like, with a special stench. Microscopic examination showed mucus with more sticky into the group of red blood cells and less white blood cells, and sometimes see Xiake - Radom crystallization and activity of trophozoites. These characteristics can be distinguished from the diarrhea of ​​bacterial diarrhea. ② cystic examination to take a small amount of bamboo stick stool sample, coated with iodine in a thin sheet coated glass, and then placed under a microscope to identify and identify the characteristics and number of nuclei.
(2) Amoeba culture due to technical operation is complicated, need certain equipment, and artificial c*tion of amoeba in the majority of subacute or chronic cases positive rate is not high, it may not be routine examination for amoebic diagnosis.
(3) histological examination by sigmoidoscopy or colonoscopy direct observation of mucosal ulcers, and for biopsy or wipe smear, the highest detection rate. Trophoblast drawing must be at the edge of the ulcer, after the clamp to take some bleeding is appropriate. Abscess puncture fluid examination in addition to note the characteristics, should be taken from the abscess wall, easier to find trophozoites.
Immunization
In recent years, many serological diagnostic methods have been reported both at home and abroad, including indirect hemagglutination (IHA), indirect immunofluorescence (IFAT) and enzyme-linked immunosorbent assay (ELISA), but the sensitivity of different types of cases . The sensitivity of IHA was higher, the positive rate of enteromycosis was 98%, the rate of extrahepatic amoebiasis was 95%, while that of asymptomatic insects was only 10% -40%. IFA sensitivity was slightly Less than IHA. EALSA is sensitive, specific and promising. In recent years, it has been reported that the detection of amebic antigen in stool and pus using a sensitive immunological technique has been reported successfully. In particular, the use of monoclonal antibodies against amoebiasis is a reliable, sensitive and anti-interference tracer for the detection of pathogenic agents in host excretions by immunological techniques.
diagnosis
The diagnosis of amebiasis, in addition to based on the patient's chief complaint, history and clinical manifestations as the basis for the diagnosis, the most important is the etiological diagnosis, detection of amebiasis in the stool is the only reliable basis for the diagnosis. Usually found to nourish people who are now as patients, and found that nodules or cysts only as infected.
Differential diagnosis
Amoeba enteropathy and bacterial diarrhea, schistosomiasis, intestinal tuberculosis, colon cancer, chronic nonspecific ulcerative colitis and other identification.
1. dysentery
Urgency, systemic symptoms of severe poisoning, antibiotic treatment is effective, stool microscopy and bacterial culture contribute to the diagnosis.
Schistosomiasis
Slow onset, long course of disease, a history of exposure to water, hepatosplenomegaly, blood eosinophilia, schistosomiasis eggs can be found in feces or hatching of cercariae, intestinal mucosa can be found in the living tissue eggs.
3. Intestinal tuberculosis
Most of the primary tuberculosis exists, the patient has a consumption of heat, night sweats, nutritional disorders, etc .; stools were mostly yellow gruel, with mucus and less pus and blood, diarrhea and constipation alternay. Gastrointestinal X-ray examination can help diagnose.
Colon cancer
Patients older, more changes in bowel habits, thinning stools, progressive anemia, weight loss. Late palpable abdominal mass, barium enema X-ray examination and colonoscopy help to diagnose.
5. Chronic nonspecific ulcerative colitis
Clinical symptoms and chronic amebiasis is not easy to distinguish, but stool examination can not be found amoeba, and anti-amoeba treatment is still not effective when the disease can be considered.