Agilent Instrument Control Framework (ICF) allows third-party data acquisition and processing software to control Agilent LCs. In this Application Note, an Agilent 1290 Infi nity II LC was controlled through Waters Empower 3 software for the separation of six β-blockers with subsequent data processing and data analysis. The precision of retention time and area for each compound was calculated to evaluate the performance of the LC system.