Rabbit  Anti-Protein A蛋白A抗体颜色, HRP conjugated

FROM:RICBIO
Catalog Number : RS-0362R-HRP 

Quantity size : 100ug (1mg/ml  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.)

background:
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.

Specificity : · Rabbit Polyclonal IgG, affinity purified by Protein A 

           · Reacts with: Human,rat

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG  

· mol wt：40-60kDa. kDa

Application : WB=1:100-500  ELISA=1:500-1000  IHC-F=1:100-500  IF=1:100-500   

Optimal working dilutions must bedetermined by the end user

Anti-Protein A蛋白A抗体颜色

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important NoteAnti-Protein A蛋白A抗体颜色: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.