DOTA-GRGDSPC，DOTA偶联多肽GRGDSPC

DOTA-GRGDSPC，DOTA偶联多肽GRGDSPC

名称：DOTA-GRGDSPC，DOTA偶联多肽GRGDSPC

一种含 7 个氨基酸的多肽，是一种末端巯基化的细胞粘附肽。GRGDSPC, a 7-amino acid peptide, is a thiolated cell adhesion peptide.

GRGDSPC通过巯基丙烯酸反应与丙烯酸葡聚糖偶联，以调节人类间充质***（hMSCs）与光交联水凝胶的相互作用。为了确定GRGDSPC肽与DEX-MAES16的结合动力学和效率，在pH 7.8的PBS中，随着时间的推移（0.25、0.5、1和3h），各种GRGDSPC-浓度（即5、10和20 mg/1 g DEX-MAES 16）与丙烯酸葡聚糖（DEX）大分子单体结合，并使用Ellman分析法量化未反应肽的游离巯基。此外，还比较了巯基肽与丙烯酸酯（DEX-MAES16）和甲基丙烯酸（DEX-HEMA16）大分子单体的反应动力学。早在15分钟接合时，用5、10和20 mg GRGDSPC肽/1 g修饰的DEX，与DEX-MAES的肽接合效率分别为105.40、94.10和87.45%，而与DEX-HEMA的反应分别为0.73、15.78和18.42%。1h后，GRGDSPC与DEX-MAES的接合在肽浓度为10mg时完成，但肽中只有35.66%的巯基与DEX-HEMA反应。反应动力学也在接合3小时时进行监测，所有20 mg GRGDSPC肽与丙烯酸DEX反应，而此时甲基丙烯酸DEX仅为32.53%。

GRGDSPC is conjugated to acrylated dextran via thiol-acrylate reaction to regulate the interactions of human mesenchymal stem cells (hMSCs) with the photocrosslinkable hydrogels. To determine the conjugation kinetics and efficiency of GRGDSPC peptide to DEX-MAES16, various GRGDSPC concentrations (i.e., 5, 10 and 20 mg/1 g DEX-MAES16) are conjugated to the acrylated Dextran (DEX) macromer over time (0.25, 0.5, 1 and 3h) in PBS at pH 7.8 and the free thiol groups of unreacted peptides are quantified using Ellman&＃39;s assay. In addition, the reaction kinetics of the thiol-peptide to acrylated (DEX-MAES16) and methacrylated (DEX-HEMA16) macromers are compared. As early as 15 min conjugation, with 5, 10 and 20 mg of GRGDSPC peptide/1 g modified DEX, the peptide conjugation efficiencies with DEX-MAES are 105.40, 94.10 and 87.45%, respectively, while for the reaction with the DEX-HEMA they are 0.73, 15.78 and 18.42%, respectively. After 1h, the GRGDSPC conjugation with DEX-MAES is completed with the peptide concentration of 10 mg, but only 35.66% of the thiol groups of the peptide react with DEX-HEMA. The reaction kinetics are also monitored at 3 h of conjugation, and all of the 20 mg GRGDSPC peptide reacts with acrylated DEX compared to only 32.53% for the methacrylated DEX at this time point.

中文名称：GRGDSPC

英文名：GRGDSPC

CAS号：91575-26-7

单字母：H2N-GRGDSPC-OH

三字母：H2N-Gly-Arg-Gly-Asp-Ser-Pro-Cys-COOH

氨基酸个数：7

分子式：C25H42N10O11S1

平均分子量：690.73

***分子量：690.28

等电点(PI)：8.55

pH=7.0时的净电荷数：0.94

平均亲水性：1.325

疏水性值：-1.24

来源：Synthetic

纯度：95%、98%

盐体系：可选TFA、HAc、HCl或其它

生成周期：2-3周  

相关试剂列表：

DOTA-FSHB    DOTA偶联卵泡刺***多肽FSHB81-95

DOTA-NYZL1    DOTA偶联膀胱癌靶向肽NYZL1

DOTA-HAIYPRH    DOTA偶联转铁蛋白靶向肽HAIYPRH

DOTA-SP94    DOTA偶联肝癌特异靶向肽SP94

DOTA-TH    DOTA偶联PH响应性细胞穿膜肽TH

DOTA-M918    DOTA偶联细胞穿膜肽M918

DOTA-YTA2    DOTA偶联细胞穿膜肽YTA2

DOTA-YTA4    DOTA偶联细胞穿膜肽YTA4

DOTA-Pep-1    DOTA偶联细胞穿膜肽Pep-1

DOTA-TAT(48-60)    DOTA偶联细胞穿膜肽TAT(48-60)

DOTA-MAP    DOTA偶联细胞穿膜肽MAP

DOTA-CADY    DOTA偶联细胞穿膜肽CADY

DOTA-pVEC    DOTA偶联细胞穿膜肽pVEC

DOTA-MPG    DOTA偶联细胞穿膜肽MPG

DOTA-TAT(48-60)    DOTA偶联细胞穿膜肽 TAT