TruSeq DNA PCR-Free LT Library

TruSeq DNA PCR-Free LT Library

Library Preparation Kits "Product Highlights:

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

Shortened gel-free workflows that remove the need for PCR

ABIlity to sequence the most challenging regions

Improved genome coverage to identify the greatest number of variants

Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are trADItionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols

Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions

Species Category Rat,Mouse

Method Whole-Transcriptome Sequencing

Technology Sequencing" 735.00 盒 "上海易汇生物科技有限公司，是一家集研发、销售为一体的生物企业，公司专注于生命科学和生物技术领域，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已代理 LKT Laboratories，MEDICOM（麦迪康），Biovision，ApexBio，阿拉丁，麦克林，TCI，Epigentek,Eaivelly,Abnova,GeneBrick等品牌。