目录:北京索莱宝科技有限公司>>ELISA试剂盒>>人 Human ELISA>> SEKH-0046Human IFN-gamma
| 供货周期 | 现货 | 规格 | 1600/48T; 2600/96T |
|---|---|---|---|
| 货号 | SEKH-0046 | 主要用途 | 检测人血清/血浆/细胞培养上清中干扰素的含量,定量检测 |
Human IFN-gamma
| 中文名称 | 人干扰素γ检测试剂盒 |
|---|---|
| 英文名称 | Human IFN-γ ELISA KIT |
| Reactivity | Human |
| Sample type | Serum, plasma, Cell culture supernatant |
| Sensitivity | 4 pg/ml |
| Detection Target | IFN-γ |
| Applications | Sandwich ELISA |
Human IFN-gamma
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm
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