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TR101Transfection reagent转染试剂

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  • 桑戈国际贸易(上海)有限公司
  • 15元(具体成交价以合同协议为准)
  • 2021-01-21 16:36:22
  • 上海市
  • 美国
  • 665

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【简单介绍】

品牌 其他品牌 供货周期 现货
应用领域 化工,生物产业,石油,电子
Transfection reagent转染试剂
Introduction to Clarkfection
Clarkfection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the

【详细说明】

Transfection reagent转染试剂

Cat No.Product NamePacking Size  
TR101Clarkfection transfection reagent1ml  

Introduction to Clarkfection

Clarkfection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Clarkfection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.

Clarkfection Features

  • For eukaryotic cells like HEK 293;
  • For both adherent and suspension cells;
  • For operations with or without serum;
  • More than 200,000 L application.

High protein expression and low cytotoxicity(EGFP protein expression transfected Clarkfection )

 

293E293FT
  COS7HepG2
BHK21MCF7
  SW480CHO-DG44

Clarkfection Transfection Protocol

The transfection procedures of adherent cells are shown in Fig. 1.
we take the 96-well plates as example:
4cells/well (table 1)and cultured with 5%CO2 at 37℃ for 18-24h before transfection.
2. Preparation of transfection mixture:
(1). Dilute DNA into serum-free medium(25μL in total) and homogenize gently.
(2). Dilute Clarkfection into serum-free medium(25μL in total) and homogenize gently and incubate at room temperature for 5 min.
(3). Mix the DNA and Clarkfection at room temperature for 15~20min 3. Remove the culture medium and add 50μL mixture per well.
4. After 4-6hours(for SF9 cell is 2h), remove the transfection mixture and add medium with serum.
5. Gene expression is tested after incubation with 5% CO2 at 37℃ for 48-72h(incubate sf9 cell line at 27℃ for 48-72h).

 

Fig. 1 Adherent cells' Transfection

Attention:

1). The experiment conditions for different cell lines are shown in Table 1. The corresponding transfection protocols can be got by entering links in Table 1.
(2). Number of cells per well, dosage of Clarkfection, DNA and serum-free medium for dilution are proportional to basal area per well for plates of different sizes. The basical areas for plates with different sizes are shown in Table 2. The dosage and ratio of DNA and Clarkfection should be optimized to achieve the best transfection results.

Table1. Usage of Clarkfection in different cell lines(96-well plate)

Cell typeCulture mediumCells per wellDNAClarkfectionMedium change after 4-6h
293HDMEM3×1040.2µg0.5µLDMEM+10%FBS
293FTDMEM3×1040.2µg0.5µLDMEM+10%FBS
293EDMEM3×1040.2µg0.5µLDMEM+10%FBS
293FDMEM3×1040.2µg0.5µLDMEM+10%FBS
COS7DMEM1.5×1040.4µg0.5µLDMEM+10%FBS
helaDMEM2×1040.3µg0.5µL1640+15%FBS
Caco2MEM3.5×1040.3µg0.75µLMEM+10%FBS
BHK21MEM2×1040.2µg0.5µLMEM+10%FBS
CHO-DG44DMEM+HT+pro2×1040.5µg0.5µLDMEM+HT+pro +10%FBS
RAW264.7DMEM3×1040.2µg0.5µLDMEM +10%FBS
MCF7MEM/NEAA+0.01mg/mL insulin + sodium pyruvat2×1040.1µg0.25µLMEM/NEAA+0.01mg/mL insulin + sodium pyruvat+10%FBS
SW480IMDM3×1040.4µg0.5µLIMDM +10%FBS
MDCKDMEM4×1040.6µg1µLDMEM+10%FBS
CHO-K1IMDM+Pro3×1040.2µg0.5µLIMDM+Pro +10%FBS
HepG2DMEM3×1040.5µg0.75µLDMEM+10%FBS
A549DMEM2×1040.3µg0.5µLDMEM+10%FBS
NIH/3T3DMEM1.5×1040.1µg0.75µLDMEM+10%FBS
veroDMEM3×1040.3µg0.75µLDMEM+10%FBS
sf9SIM SF5×1040.4µg0.75µLSIM SF+10%FBS

Table 2. Scaling up or down transfections with Clarkfection(according to the growth area).
Note: the actual conditions should be optimized by experiments.

 

    
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