高通量高分辨率细胞电刺激培养成像仪

MaxTwo高通量高分辨率细胞电刺激培养成像仪

MaxTwo, a high-throughput, high-resolution electrophysiology platform for recording and stimulating electrogenic cells in vitro. MaxTwo combines 6 or 24 MaxOne high-density microelectrode arrays in a multiwell format.

Product Overview

MaxWell Biosystems’ MaxTwo is a CMOS-based multiwell HD-MEA platform. It has all the capabilities of MaxOne–it captures high-quality signals at sub cellular resolution from long-term culture experiments. Electrical stimulation protocols can also be performed.

MaxTwo multiwell HD-MEA system allows for easy and vibration-free insertion of the MEA device. It can operate inside cell-culture incubators and is designed for integration with automation systems. Microscopy of samples in MaxTwo wells can be performed using upright objectives. Overall, MaxTwo is a powerful electrical imaging system for drug discovery, safety pharmacology, and iPSC phenotyping applications.

Key Features per Well

• 26,400 electrodes (9.3×5.45 sq-μm, 17.5 μm pitch)
• 1024 low-noise readout channels
• 32 stimulation channels
• Large sensor area (3.85×2.10 sq-mm)
• Switch-matrix technology for flexible array reconfiguration
• Non-invasive, label-free
• High-resolution activity map
• Axonal action potential propagation tracking
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MaxOne是记录和刺激体外细胞的zui强大的电生理平台。 MaxOne是单孔格式的高密度微电极阵列（HD-MEA）系统。
MaxOne是一款基于CMOS的高密度微电极阵列（HD-MEA）系统。
这使得紧凑而又功能强大的放大器，滤波器和数字化仪能够集成到MEA中，并且与待研究的细胞紧密相连。

高分辨率，无标记细胞活动成像
亚细胞和网络活动的无标记检测
多种选择性电刺激
全面的数据分析工具

从原发组织中收集细胞，如大脑，视网膜或心脏。

准备实验时，开始在MaxWell Biosystems的MEA芯片上培养细胞。

开始您的实验：在MaxLab Live软件的帮助下，跟踪结果并在计算机上分析数据。

使用结果来指导进一步的实验，获得科学见解并发布数据。

Multiscale recording and analysis —

1. High-resolution, label-free cell activity imaging

Analyze subcellular features, such as full axonal arbors in single neurons.

Electrical signals tracked along neurites enable to investigate novel parameters. High-resolution tracking of axonal action potential propagation allows for investigating changes in axonal conduction velocity.

2. Whole sample activity map

Localize active cells on the MEA during experiments.

The morphological structure of a primary cortical cell culture was imaged using an upright microscope with 10x objective. MAP2 staining was used to visualize the neurons. The optical image closely matches the electrical image obtained using MaxOne. The electrical image provides the location of the cells, as well as information on the activity of the cells, such as spike rate and amplitude.

3. Smart population recording

Identify defined cells for long term recording and further analyses.

Different combinations of parameters can be used to automatically select a defined set of cells to be recorded, such as spike rate, amplitude, etc. The raster plot shows the dynamics of the network activity using 1,024 active electrode sites. Burst features can be investigated in detail (one burst zoomed in).