B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书_invitrogen-上海恪敏生物科技有限公司

描述

B-27® 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体，可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。

B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用，我们提供其他配方的B-27® 添加剂.

质量控制检测
B-27® 添加剂进行了18天妊娠的原代大鼠（Sprague Dawley）的胚胎海马神经元生长检测。通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。

仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。

详细说明

常用规格

Cell Type:	Neuronal Cells
Product Size:	10 ml
Classification:	Serum-Free
Other Additives:	Vitamin A, Antioxidant Cocktail, Insulin
Purity or Quality Grade:	Cell Culture Grade
Green Features:	Sustainable packaging
Shipping Condition:	Dry Ice
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

引文和参考文献（17）

D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者: Congar P; Bergevin A; Trudeau LE
杂志: J Neurophysiol （2002） 87:1046-1102
产品用途: Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）.
ID:: PN64425

Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者: Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;
杂志: Neuron （2002） 33:789-804
产品用途: Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.
ID:: PN47418

Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.

作者: Kabos Peter; Kabosova Andrea; Neuman Toomas;
杂志: J Biol Chem （2002） 277:8763-8769
产品用途: Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）
ID:: PN63471

Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者: Lesuisse Christian; Martin Lee J;
杂志: J Neurobiol （2002） 51:9-23
产品用途: A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.
ID:: PN60461

An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者: Joseph-Bravo Patricia; PÃ©rez-MartÃnez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;
杂志: Brain Res Brain Res Protoc （2002） 9:93-104
产品用途: Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties
ID:: PN62028

更多..

回到页首

按细胞系排列的引文

细胞系名称	有机体类型	组织
SH-Sy5Y	Human	Brain
Fetal neurons	Rat	Brain
Neurons （post-natal/adult）	Rat	Brain
Neurons （superior cervical ganglia - SCG）	Rat	Brain
Neurons （cortical）	Mouse	Other
Neural Stem Cells	Rat	Brain

主营：生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、标准溶液……

abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……

以下为本公司常备现货产品

*部分 invitrogen 原装产品

品牌	货号	名称	规格
invitrogen	13778-075	Lipofectamine RNAiMAX	0.75ml
invitrogen	13778-150	Lipofectamine RNAiMAX	1.5ml
invitrogen	11668-027	Lipofectamine 2000 Reagent	0.75ml
invitrogen	11668-019	Lipofectamine 2000 Reagent	1.5ml
invitrogen	15596-026	TRlzol ®Reagent	100ml
invitrogen	15596-018	TRlzol ®Reagent	200ml
invitrogen	10296-010	Trizol ® LS Reagent	100ml
invitrogen	10296-028	Trizol ® LS Reagent	200ml
invitrogen	17504-044	B-27（黄颜色）	10ml
invitrogen	12587-010	B-27（黄颜色）不含维生素A	10ml
invitrogen	17502-048	N-2 （粉红颜色）	5ml
invitrogen	18064-014	SuperScript® II Reverse Transcriptase	10,000U
invitrogen	18080-044	SuperScript® III Reverse Transcriptase	10,000U

第二部分 HyClone胎牛血清

品牌	货号	名称	规格
HyClone	SH30370.03	Australian Standard Fetal Bovine Serum	500ml
HyClone	SH30406.02	FOETAL BOVINE SERUM	500ml
HyClone	SH30401.01	New Zealand Calf Serum	500ml
HyClone	SH30066.02	FetalClone® II	100ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30118.02	Newborn Bovine Calf Serum, U.S. Origin	100ml
HyClone	SH30070.02	FETAL BOVINE SERUM	100ml
HyClone	SH30087.02	COSMIC CALF® SERUM	100ml
HyClone	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED	500ml
100ml	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED
HyClone	SH30070.03	FETAL BOVINE SERUM	500ml
HyClone	SH30080.03	FetalClone® I	500ml
HyClone	SH30084.03	FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN	500ml
HyClone	SH30396.03	FETAL BOVINE SERUM CHARACTERIZED	500ml
HyClone	SV30087.02	南美源胎牛血清	500ml
HyClone	sv30087.01	南美源胎牛血清	100ml
HyClone	SH30071.03	FETAL BOVINE SERUM	500ml
HyClone	SH30071.02	FETAL BOVINE SERUM	100ml
HyClone	SH30080.02	FetalClone® I	100ml
HyClone	SH30070.02E	FETAL BOVINE SERUM CHARACTERIZSED	100ml
HyClone	SH30079.02	FETAL BOVINE SERUM	100ml
HyClone	SH30068.02	CHARCOAL/DEXTRAN TREATED FBS，活性炭灭活血清	100ml
HyClone	SH30068.03	CHARCOAL/DEXTRAN TREATED FBS，活性炭灭活血清	500ml
HyClone	SH30066.03	FetalClone® II	500ml
HyClone	SH30109.03	HyClone FetalClone® III	500ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30088.02	STANDARD FETAL BOVINE SERUM	100ml
HyClone	SH30079.03	FETAL BOVINE SERUM	500ml

第二部分 Gibco胎牛血清

品牌	货号	名称	规格
Gibco	12662-029	MSC QUALIFIED FBS 500ML	500ML
Gibco	10438-026	FBS, HI 500ML	500ML
Gibco	10439-024	FBS, ES CELL QUAL. USDA APPD. 500ML	500ML
Gibco	10099-141	FBS, AUSTRALIAN ORIGIN 500ML	500ML
Gibco	26400-044	FBS, DIALYZED, USA ORIGIN 500ML	500ML
Gibco	26140-079	FBS QUALIFIED, USA ORIGIN 500ML	500ML
Gibco	16141-079	FBS, ES CELL QUAL, USA ORIGIN 500ML	500ML
Gibco	16140-071	FBS, HI 500ML	500ML
Gibco	16000-044	FBS, CERT, USA ORIGIN 500ML	500ML
Gibco	C2027050	FBS, URUGUAYAN ORIGIN	500ML
Gibco	12657-029	巴西胎牛	500ml

B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书返回列表页

描述

详细说明

常用规格

引文和参考文献（17）

D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.

Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

按细胞系排列的引文

化工仪器网

收藏该商铺

提示

B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书 返回列表页

描述

详细说明

常用规格

引文和参考文献 （17）

D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.

Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

按细胞系排列的引文

化工仪器网

会员登录

收藏该商铺

提示

客户评价列表

B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书返回列表页

引文和参考文献（17）