微生物冻干保护剂
- 公司名称 博医康（北京）仪器有限公司
- 品牌
- 型号
- 产地 北京
- 厂商性质 生产厂家
- 更新时间 2023/11/27 11:03:35
- 访问次数 2784
联系方式：叶明徽010-68173889 查看联系方式
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该保护剂不含动物蛋白质！，微生物培养液与保护剂混合即可使用。使用该保护剂，细菌的存活率在90 ％以上，远远超过使用蔗糖和脱脂牛奶方法的细菌存活率。该试剂无菌过滤处理，包装是100毫升PET瓶。
使用方法：
1. Simple Culture Preservation:
Add an appropriate volume Lyophilized Buffer to a sterile vial(e.g., 100-500ul). Inoculate the buffer with 10ul of actively growing bacteria. All procedures must be performed aseptically.
2.Preservation of Large Number of Cells:
Pellet cells from an actively growing culture by centrifugation. Decant the medium and resuspend the cell in an equal volume of Lyophilized Buffer. Transfer the cell solution to a sterile vial.
The freeze drying parameters must be determined for each freeze dryer. However the buffer are
optimized to freeze drying on the following cycle.
Freezing:30 minutes from ambient to -40℃, hold for 1 hr.
Primary Drying: Increase temperature to -10℃, hold for 16 hours(large volumes
may require longer reying times).
Secondary Drying:Increase temperature to 20℃, hold for 2 hr.
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